Stemgent® DyLight™ 549 Mouse anti-Human Tra-1-81 Antibody
产品名称： Stemgent® DyLight™ 549 Mouse anti-Human Tra-1-81 Antibody
英文名称： Stemgent® DyLight™ 549 Mouse anti-Human Tra-1-81 Antibody
产品编号： 09-0082
产品价格： null
产品产地： USA
品牌商标： Stemgent
更新时间： 2023-08-17T13:40:59
使用范围：
- 联系人 : 李自转
- 地址 : 上海市浦东新区天雄路166弄一号楼三层南单元
- 邮编 : 200030
- 所在区域 : 上海
- 电话 : 139****5640
- 传真 : 021-34615995-188
- 邮箱 : lizizhuan@yeasen.com
Product Overview
Stemgent® DyLight™ 549 Mouse anti-Human TRA-1-81 Antibody was screened on human embryonic stem (ES) cells using immunocytochemistry (ICC). The TRA-1-81 monoclonal antibody reacts with a pluripotent stem cell-specific antigen expressed upon the surface of human embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-81 antigen is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. As human EC and ES cells undergo differentiation, expression of TRA-1-81 antigen is lost. TRA-1-81 antibody recognizes a carbohydrate epitope expressed on podocalyxin, a sialoglycoprotein structurally related to CD34. Podocalyxin is a transmembrane glycoprotein which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.
References
- Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. (2007). Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors. Cell 131, 861-872.
- Yu J., Vodyanik M.A., Smuga-Otto K., Antosiewicz-Bourget J., Frane J.L., Tian S., Nie J., Jonsdottir G.A., Ruotti V., Stewart R., Slukvin I.I., Thomson J.A. (2007). Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917-1920.
- Schopperle W.M., DeWolf W.C. (2007). The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25, 723-730.
- Chin A.C., Fong W.J., Goh L.T., Philp R., Oh S.K., Choo A.B. (2007) Identification of proteins from feeder conditioned medium that support human embryonic stem cells. J Biotechnol. 130, 320-328.
- Andrews PW, Banting G, Damjanov I, Arnaud D, Avner P. (1984). Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3, 347.
- Lowry, W.E., Richter, L., Yachechko, R., Pyle, A.D., Tchieu, J., Sridharan, R., Clark, A.T., Plath, K. (2008) Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A 105: 2883-8.
Product Specifications
| Immunogen | Human embryonal carcinoma cell line 2102Ep |
| Reactivity | Human |
| Clone | TRA-1-81 |
| Isotype | Mouse IgM,κ |
| Formulation | Phosphate-buffered solution pH 7.2 containing ≤0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with Dylight™ 549 under optimal conditions. The solution is free of unconjugated dye and free antibody. |
| Storage | Store undiluted at 4oC. Do not freeze. Light sensitive. |
| Stability | Stable for 6 months from date of receipt when stored as directed. |
| Quality Control | Tested by immunocytochemistry to ensure product quality. |
| Recommended Usage | Use antibody at 1:100 in ICC. For general protocols for immunocytochemistry on stem cells, see the Technical Documents on the Stemgent® Dylight™ 549 Mouse anti-Human Tra-1-81 product page. |
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| Trademark | DyLight™ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. |