TPPII抗体Applications
| In-house applications |
ELISA, WB, IHC, IF, FC, IP Application not tested? Please see our Guarantee. |
| Published applications |
IF |
Publications |
1 citations –view here |
| Species specificity |
Human, Mouse; other species not tested. |
| Published Species |
Human |
|
Images
Prev12Next
 Jurkat cells were subjected to SDS PAGE followed by western blot with 14120-1-AP(TPP2 antibody) at dilution of 1:1000 Immunohistochemical of paraffin-embedded human gliomas using 14120-1-AP(TPP2 antibody) at dilution of 1:100 (under 10x lens) Immunohistochemical of paraffin-embedded human gliomas using 14120-1-AP(TPP2 antibody) at dilution of 1:100 (under 40x lens) Immunofluorescent analysis of HepG2 cells, using TPP2 antibody 14120-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). IP Result of anti-TPP2 (IP:14120-1-AP, 4ug; Detection:66017-1-Ig, 1:500) with Jurkat cells lysate 2400ug. 1X10^6 HepG2 cells were stained with 0.2ug TPP2 antibody (14120-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
|
| Positive WB detected in |
Jurkat cells, K-562 cells |
| Positive IP detected in |
Jurkat cells |
| Positive IHC detected in |
Human gliomas tissue |
| Positive IF detected in |
HepG2 cells |
| Positive FC detected in |
HepG2 cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200 |
TPPII抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information TPPII抗体
| Immunogen |
TPPII fusion protein ag5296 |
Full name |
Tripeptidyl peptidase II |
Calculated molecular
weight |
1249aa,138 kDa |
Observed molecular
weight |
135-140 kDa |
GenBank accession
number |
BC039905 |
Gene ID (NCBI) |
7174 |
| Gene symbol |
TPP2 |
Synonyms |
FLJ40359, TPP 2, TPP II, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, Tripeptidyl peptidase II |
Background
|
TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022). TPPII抗体
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